期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 131, 期 12, 页码 4301-4309出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja807503g
关键词
-
资金
- Singapore Ministry of Education [ARC30/07]
- Nanyang Technological University (NTU) [SUG5/06, RG138/06, M58110032]
- Singapore Ministry of Education AcRF Tier 2 [T206B321ORS]
- University Research Committee grant [RG65/06]
- U.S. National Institutes of Health [GM34504]
Previously, it has been reported that human telomeric DNA sequences could adopt in different experimental conditions four different intramolecular G-quadruplexes each involving three G-tetrad layers, namely, Na+ solution antiparallel-stranded basket form, K+ crystal parallel-stranded propeller form, K+ solution (3 + 1) Form 1, and K+ solution (3 + 1) Form 2. Here we present a new intramolecular G-quadruplex adopted by a four-repeat human telomeric sequence in K+ solution (Form 3). This structure is a baskettype G-quadruplex with only two G-tetrad layers: loops are successively edgewise, diagonal, and edgewise; glycosiclic conformations of guanines are syn center dot syn center dot anti center dot anti around each tetrad. Each strand of the core has both a parallel and an antiparallel adjacent strands; there are one narrow, one wide, and two medium grooves. Despite the presence of only two G-tetrads in the core, this structure is more stable than the three-G-tetrad intramolecular G-quadruplexes previously observed for human telomeric sequences in K+ solution. Detailed structural elucidation of Form 3 revealed extensive base pairing and stacking in the loops capping both ends of the G-tetrad core, which might explain the high stability of the structure. This novel structure highlights the conformational heterogeneity of human telomeric DNA. It establishes a new folding principle for G-quadruplexes and suggests new loop sequences and structures for targeting in human telomeric DNA.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据