期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 131, 期 36, 页码 12960-12969出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja9016864
关键词
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资金
- NSF [CHE-0130903]
- NIH [U54 RR022241]
- ARCS
- NDSEG
Fluoromodules are discrete complexes of biomolecules and fluorogenic dyes. Binding of the dyes to their cognate biomolecule partners results in enhanced dye fluorescence. We exploited a previously reported promiscuous binding interaction between a single-chain, variable fragment antibody protein and a family of cyanine dyes to create new protein-dye fluoromodules that exhibit enhanced photostability while retaining high affinity protein-dye binding. Modifications to the dye structure included electron-withdrawing groups that provide resistance to photo-oxidative damage. Low nanomolar equilibrium dissociation constants were found for the new dyes. Fluorescence microscopy illustrates how yeast can be surface-labeled with three different colors based on a single protein and appropriately chosen dyes.
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