期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 131, 期 23, 页码 8308-8312出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja9017013
关键词
-
资金
- Institute for Solid State Physics
- The University of Tokyo
- Japanese Ministry of Education, Culture, Sports, Science and Technology [17390011, 20050017, 20790032]
- Fundamental Studies in Health Science
- National Institute of Biomedical Innovation (NIBIO)
- Grants-in-Aid for Scientific Research [20050017, 20790032, 17390011] Funding Source: KAKEN
Nanodiscs are phospholipid-protein complexes which are relevant to nascent high-density lipoprotein and are applicable as a drug carrier and a tool to immobilize membrane proteins. We evaluated the structure and dynamics of the nanoparticles consisting of dimyristoylphosphatidylcholine (DMPC) and apolipoprotein A-I (apoA-I) with small-angle neutron scattering (SANS) and fluorescence methods and compared them with static/dynamic properties for large unilamellar vesicles. SANS revealed that the nanodisc includes a lipid bilayer with a thickness of 44 angstrom and a radius of 37 angstrom, in which each lipid occupies a smaller area than the reported molecular area of DMPC in vesicles. Fluorescence measurements suggested that DMPC possesses a lower entropy in nanodiscs than in vesicles, because apoA-I molecules, which surround the bilayer, force closer lipid packing, but allow water penetration to the acyl chain ends. Time-resolved SANS experiments revealed that nanodiscs represent a 20-fold higher lipid transfer via an entropically favorable process. The results put forward a conjunction of static/dynamic properties of nanodiscs, where the entropic constraints are responsible for the accelerated desorption of lipids.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据