期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 131, 期 22, 页码 7755-7761出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja901294r
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资金
- BBSRC [BB/E004350/1] Funding Source: UKRI
- EPSRC [EP/G026688/1, EP/E000614/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [EGA17763, BB/E004350/1] Funding Source: Medline
- Biotechnology and Biological Sciences Research Council [BB/C510824/1, BB/E004350/1, EGA17763] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/D023335/1, GR/T26542/01, EP/E000614/1, EP/G026688/1, EP/D023343/1] Funding Source: researchfish
We describe microarraying of carbohydrates for protein screening using either disulfide bridge or Schiff base imine immobilization chemistries on plasmachemical deposited functional nanolayers. The commonly observed issue of nonspecific background binding of proteins is overcome by spotting carbohydrates through a protein-resistant overlayer yielding spatially localized interaction with a reactive functional underlayer.
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