期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 131, 期 50, 页码 18054-+出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja905623k
关键词
-
资金
- The Icelandic Research Fund [080041022]
- Eimskip Fund of the University of Iceland
The spin-labeled nucleoside C-T, containing 2,2,6,6-tetramethyipiperidine-l-oxyL (TEMPO) conjugated to the exocyclic amino group of C, was used to detect single-base mismatches in duplex DNA for the first time by electron paramagnetic resonance (EPR) spectroscopy. Furthermore, the EPR spectra of the fully base-paired duplex (TC-G) and the mismatches (C-T-A, C-T-C, and C-T-T) were significantly different, showing that the probe can identify its base-pairing partner in DNA. At lower pH, the mobility of C-T-A, TC.C, and C-T-T became higher, consistent with increased protonation of the mismatched pairs. Although the duplexes for each of the three flanking sequences tested gave distinguishable EPR signals, the best discrimination between base pairs was achieved for sequences containing a flanking A-T base pair, in particular 5'-d(G(T)CA) and 5'-d(IICA). This study shows that minor structural variations in nucleic acids can be detected with carefully chosen spin [abets in conjunction with EPR spectroscopy.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据