Selective Recognition of Unnatural Imidazopyridopyrimidine:Naphthyridine Base Pairs Consisting of Four Hydrogen Bonds by the Klenow Fragment

In this work, we investigated how thermally stable ImO(N):NaN degrees and ImN degrees:NaO(N) pairs are recognized by the Klenow fragment (KF). As a result, these complementary base pairs, especially the ImN degrees:NaO(N) pair, were recognized selectively due to the four hydrogen bonds between the nucleobases and the shape complementarity of the Im:Na pair similar to the purine:pyrimidine base pair.