期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 131, 期 43, 页码 15761-15768出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja9052027
关键词
-
资金
- Grant Agency of AS CR [KAN200100801, Z60220518]
- Center for Biomolecular Magnetic Resonance (BMRZ)
- Cluster of Excellence Frankfurt Macromolecular Complexes (CEF)
- EC [R113-026145]
In-cell NMR spectroscopy of proteins in different cellular environments is a well-established technique that, however, has not been applied to nucleic acids so far. Here, we show that isotopically labeled. DNA and RNA can be observed inside the eukaryortic environment of Xenopus laevis oocytes by in-cell NMR spectroscopy. One limiting factor for the observation of nucleic acids in Xenopus oocytes is their reduced stability. We demonstrate that chemical modification of DNA and RNA can protect them from degradation and can significantly enhance their lifetime. Finally, we show that the imino region of the NMR spectrum is devoid of any oocyte background signals enabling the detection even of isotopically nonlabeled molecules.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据