期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 131, 期 36, 页码 13093-13098出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja903768f
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资金
- University Grant Council of the Hong Kong government [RGC HKUST 604606]
- National Science Foundation (NSF)
- Army Research Office (ARO)
- Arizona State University
- Air Force Office of Scientific Research
- Office of Naval Research
- National Institutes of Health
- Alfred P. Sloan Foundation
We report the design and construction of a nanometer-sized tetrahedron from a single strand of DNA that is 286 nucleotides long. The formation of the tetrahedron was verified by restriction enzyme digestion, Ferguson analysis, and atomic force microscopy (AFM) imaging. We further demonstrate that synthesis of the tetrahedron can be easily scaled up through in vivo replication using standard molecular cloning techniques. We found that the in vivo replication efficiency of the tetrahedron is significantly higher in comparison to in vitro replication using rolling-circle amplification (RCA). Our results suggest that it is now possible to design and replicate increasingly complex, single-stranded DNA nanostructures in vivo.
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