期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 130, 期 23, 页码 7182-+出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja8008924
关键词
-
资金
- Direct For Education and Human Resources
- Division Of Human Resource Development [833180] Funding Source: National Science Foundation
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [717518] Funding Source: National Science Foundation
- Howard Hughes Medical Institute Funding Source: Medline
- NHGRI NIH HHS [P50 HG002806] Funding Source: Medline
Here we report on a phenanthridine derivative which has a covalently linked fluorescein molecule in order to increase the light absorption and hence fluorescence signal intensity when bound to duplex RNA. Steady-state fluorescence shows that the energy transfer efficiency from the fluorescein to the phenanthridine fluorophore is approximately 77%, which results in the probe being over 5x brighter than other phenanthridine derivatives when bound to RNA. Due to the relatively long lifetime (-20 ns) of the probe, time-resolved fluorescence is used to increase the signal to background ratio in cell growth medium from 7 (steady-state value) to over 40. Moreover, fluorescence images of cells containing the probe show that the fluorescein signal is readily apparent along with that of the intercalated fluorophore, allowing this probe to be used as a dual color probe which simultaneously reports the probes' location and that of RNA.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据