期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 130, 期 46, 页码 15543-15548出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja804968g
关键词
-
资金
- Clarendon Scholarship in partnership with a Lady Noon Award
- Royal Society Wolfson Research Merit Award
- Medical Research Council
- National Institutes of Health
- MRC [G0300122] Funding Source: UKRI
- Medical Research Council [G0300122] Funding Source: researchfish
Droplet interface bilayers (DIBs) form between two lipid monolayer-encased aqueous droplets submerged in oil. Both major structural classes of membrane proteins, alpha-helix bundles and beta barrels, represented by channels and pores, respectively, spontaneously insert into DIBs when freshly expressed by cell-free transcription and translation. Electrodes embedded within the droplets allow the measurement of transmembrane ionic currents carried by individual channels and pores. On the basis of these findings, we have devised a chip-based approach for the rapid screening of blockers against ion channels. The technique is demonstrated here with the viral potassium channel, Kcv.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据