期刊
PLOS ONE
卷 10, 期 6, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0128718
关键词
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资金
- Funds of Leading Talents of Guangdong
- Programme of Introducing Talents of Discipline to Universities [B14036]
- Project of International
- Taiwan Science and Technology Cooperation Innovation Platform in Universities in Guangdong Province [2013gjhz0002]
- National Program on Key Basic Research Project of China (973 Program) [2011CB707501]
- Hundred Talents program
- Qing Lan Project of Nanjing Normal University
- Jiangsu Provincial Natural Science Foundation [BK20140917]
- Hong Kong, Macao
Retrograde labelling of retinal ganglion cells with optic nerve transection often leads to degeneration of ganglion cells in prolonged experiments. Here we report that an intact optic nerve could uptake retrograde tracers applied onto the surface of the nerve, leading to high efficiency labelling of ganglion cells in the retina with long-term survival of cells. This method labelled a similar number of ganglion cells (2289 +/- 174 at 2 days) as the retrograde labeling technique from the superior colliculus (2250 +/- 94) or optic nerve stump (2279 +/- 114) after transection. This finding provides an alternative way to label retinal ganglion cells without damaging the optic tract. This will facilitate anatomical studies in identifying the morphology and connectivity of retinal ganglion cells, allowing secondary or triple labelling manipulations for long-term investigations.
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