期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 130, 期 29, 页码 9204-+出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja802883k
关键词
-
资金
- NHGRI NIH HHS [P20 HG003638, P20 HG003638-02, P20-HG003638-02] Funding Source: Medline
We have reengineered a red-emitting dicyanomethylenedihydrofuran push-pull fluorophore so that it is dark until photoactivated with a short burst of low-intensity violet light. Photoactivation of the dark fluorogen leads to conversion of an azide to an amine, which shifts the absorption to long wavelengths. After photoactivation, the fluorophore is bright and photostable enough to be imaged on the single-molecule level in living cells. This proof-of-principle demonstration provides a new class of bright photoactivatable fluorophores, as are needed for super-resolution imaging schemes that require active control of single molecule emission.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据