期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 130, 期 4, 页码 1328-1334出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja075937f
关键词
-
资金
- NCI NIH HHS [CA112075, R01 CA112075-04, R01 CA112075] Funding Source: Medline
- NIBIB NIH HHS [EB000432, R01 EB000432-05, R01 EB000432] Funding Source: Medline
Self-assembled protein capsids have gained attention as a promising class of nanoparticles for biomedical applications due to their monodisperse nature and versatile genetic and chemical tailorability. To determine the plasma clearance and tissue distribution in mice of the versatile capsid of bacteriophage Q beta, the particles were decorated with gadolinium complexes using the Cu-1-mediated azide-alkyne cycloaddition reaction. Interior surface labeling was engineered by the introduction of an azide-containing unnatural amino acid into the coat protein for the first time. Clearance rates were conveniently monitored by quantitative detection of Gd using inductively coupled plasma optical emission spectroscopy and were found to be inversely proportional to the number of complexes attached to the exterior surface of the particle. This phenomenon was correlated to changes in exterior surface charge brought about by acylation of surface-exposed amine groups in the initial step of the bioconjugation protocol. When primary amine groups were reintroduced by azide-alkyne coupling, the circulation time increased accordingly. These results show that nanoparticle trafficking may be tailored in predictable ways by chemical and genetic modifications that modulate surface charge.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据