Toward β-amino acid proteins:: Design, synthesis, and characterization of a fifteen kilodalton β-peptide tetramer

A 28 residue 'retro' beta-peptide has been designed to self-assemble as a tetramer, successfully recapitulating the octameric beta-peptide bundle complex Zwit-1F. Z28, the largest beta-peptide synthesized to date, was achieved in a linear, microwave-assisted synthesis in 19% isolated yield and high purity. Z28 forms the expected tetrameric complex and is more stable and cooperative in its folding than Zwit-1F. The successful design and synthesis of Z28 is an important step toward true R amino acid proteins.