期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 130, 期 44, 页码 14376-+出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja800322b
关键词
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资金
- MEXT of Japan
- JST
- Grants-in-Aid for Scientific Research [20675004] Funding Source: KAKEN
Lanthanide-based luminescent sensors are noteworthy because their long-lifetime luminescence enables time-resolved fluorescence measurements. After exploring suitable antenna groups, we designed and synthesized lanthanide-based luminogenic sensors detecting protease activities. This sensor yielded strong luminescence on addition of proteases such as calpain I and leucine aminopeptidase (LAP). Since the luminescence lifetimes of the probes were very long, the sensors could be applied to time-resolved measurements that exclude background fluorescence signals derived from protiens or other impurities. This sensing principle could be applicable to general time-resolved fluorescence assays for other proteases.
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