期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 130, 期 10, 页码 2924-2925出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja7106756
关键词
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资金
- NIGMS NIH HHS [GM61077, R01 GM061077-08, R01 GM061077] Funding Source: Medline
A simple method for the electrochemical detection of TATA-binding protein is demonstrated at DNA-modified microelectrodes. The assay is general and based on the interruption of DNA-mediated charge transport to Nile Blue, a redox-active probe covalently attached to the DNA base pair stack. Nanomolar quantities of TATA binding protein can be detected on the microelectrodes even in the presence of micromolar amounts of bovine serum albumin, Endonuclease III, or Bam HI methyltransferase. The scheme outlined provides a basis for the sensitive electrical detection of numerous proteins on a single DNA chip.
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