期刊
PLOS ONE
卷 10, 期 5, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0126571
关键词
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资金
- Cancer Prevention & Research Institute of Texas [RP110360]
- National Institute of Allergy and Infectious Diseases
- National Institutes of Health [AI057156]
- RCE Programs Office, NIAID
- NIH
- National Science Foundation [0840889]
- Olle Engkvist Byggmastare Foundation
- Direct For Education and Human Resources
- Division Of Graduate Education [0840889] Funding Source: National Science Foundation
Noroviruses are recognized worldwide as the principal cause of acute, non-bacterial gastroenteritis, resulting in 19-21 million cases of disease every year in the United States. Noroviruses have a very low infectious dose, a short incubation period, high resistance to traditional disinfection techniques and multiple modes of transmission, making early, point-of-care detection essential for controlling the spread of the disease. The traditional diagnostic tools, electron microscopy, RT-PCR and ELISA require sophisticated and expensive instrumentation, and are considered too laborious and slow to be useful during severe outbreaks. In this paper we describe the development of a new, rapid and sensitive lateral-flow assay using labeled phage particles for the detection of the prototypical norovirus GI. 1 (Norwalk), with a limit of detection of 107 virus-like particles per mL, one hundredfold lower than a conventional gold nanoparticle lateral-flow assay using the same antibody pair.
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