期刊
PLASMID
卷 80, 期 -, 页码 8-15出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.plasmid.2015.03.002
关键词
Aggregation phenotype; B. thuringiensis; Conjugation; Large plasmid
资金
- National Foundation for Scientific Research (FNRS)
- Universite Catholique de Louvain
- Research Department of the Communaute francaise de Belgique (Concerted Research Action, ARC)
Bacillus thuringiensis strains usually harbor large sets of plasmids, some of which carrying the entomopathogenic delta-endotoxins. B. thuringiensis serovar israelensis, active on Dipteran larvae, carries the very large conjugative plasmid pXO16 (350 kb). pXO16 displays a macroscopic aggregation phenotype when liquid cultures of conjugative partners are mixed. Its conjugative apparatus is able of transferring itself and other non-conjugative and non-mobilizable plasmids in a fast and very efficient manner. Even though its conjugative kinetics and capabilities have been extensively studied, the genetic bases for this unique transfer system remain largely unknown. In this work, the sequence of pXO16 has been identified in the existing sequenced genome of B. thuringiensis sv. israelensis HD-789 as corresponding to the p01 plasmid. Despite pXO16 sequence being highly coding, few CDS possess homologs in the databases. However, potential regions responsible for the aggregation phenotype and the plasmid replication have been highlighted. The common orientation of all CDS and the presence of a high number of potential paralogs suggested a phage-like nature. Concerning conjugative functions, no significant type IV secretion system homologs have been found, indicating that pXO16 encodes an unforeseen conjugative system. (C) 2015 Elsevier Inc. All rights reserved.
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