4.2 Article

The LaueUtil toolkit for Laue photocrystallography. II. Spot finding and integration

期刊

JOURNAL OF SYNCHROTRON RADIATION
卷 19, 期 -, 页码 637-646

出版社

INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S0909049512022637

关键词

photocrystallography; X-ray diffraction; X-ray detection; CCD; Laue method; spot integration; background estimate; statistical analysis; image filtering

资金

  1. National Science Foundation [CHE0843922]
  2. National Institutes of Health, National Center for Research Resources [RR007707]
  3. US Department of Energy, Office of Basic Energy Sciences [W-31-109-ENG-38]
  4. Direct For Mathematical & Physical Scien
  5. Division Of Chemistry [1213223, 0843922] Funding Source: National Science Foundation

向作者/读者索取更多资源

A spot-integration method is described which does not require prior indexing of the reflections. It is based on statistical analysis of the values from each of the pixels on successive frames, followed for each frame by morphological analysis to identify clusters of high value pixels which form an appropriate mask corresponding to a reflection peak. The method does not require prior assumptions such as fitting of a profile or definition of an integration box. The results are compared with those of the seed-skewness method which is based on minimizing the skewness of the intensity distribution within a peak's integration box. Applications in Laue photocrystallography are presented.

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