期刊
JOURNAL OF SYNCHROTRON RADIATION
卷 16, 期 -, 页码 133-142出版社
WILEY-BLACKWELL PUBLISHING, INC
DOI: 10.1107/S0909049509004361
关键词
radiation damage; minimum crystal size; protein macromolecular crystallography; dose doubling; radioprotectant; data collection strategy
资金
- National Institutes of Health [GM074929, GM082250]
- US Department of Energy [DE-AC03-76SF00098]
- Lawrence Berkeley National Laboratory
Many advances in the understanding of radiation damage to protein crystals, particularly at cryogenic temperatures, have been made in recent years, but with this comes an expanding literature, and, to the new breed of protein crystallographer who is not really interested in X-ray physics or radiation chemistry but just wants to solve a biologically relevant structure, the technical nature and breadth of this literature can be daunting. The purpose of this paper is to serve as a rough guide to radiation damage issues, and to provide references to the more exacting and detailed work. No attempt has been made to report precise numbers (a factor of two is considered satisfactory), and, since there are aspects of radiation damage that are demonstrably unpredictable, the 'worst case scenario' as well as the 'average crystal' are discussed in terms of the practicalities of data collection.
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