期刊
JOURNAL OF SURGICAL RESEARCH
卷 190, 期 1, 页码 55-63出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jss.2014.03.036
关键词
Oridonin; Liver fibrosis; Stellate cells; Apoptosis
类别
资金
- NCI NIH HHS [P50 CA097007] Funding Source: Medline
- NIDA NIH HHS [P30 DA028821] Funding Source: Medline
- NIDDK NIH HHS [T32 DK007639] Funding Source: Medline
- NIMH NIH HHS [R21 MH093844] Funding Source: Medline
Background: Liver fibrosis is a common response to liver injury and, in severe cases, leads to cirrhosis. The hepatic stellate cells (HSCs) become activated after liver injury and play a significant role in fibrogenesis. The activated HSC is characterized by increased proliferation, overexpression of a smooth muscle actin, and excessive production of extracellular matrix (ECM) proteins. Oridonin, a naturally occurring diterpenoid, has been shown to induce apoptosis in liver and gastric cancer cells. However, its effects on the HSC are unknown. Methods: We tested the effects of oridonin on the activated human and rat HSC lines LX-2 and HSC-T6, and the human hepatocyte cell line C3A. Transforming growth factor beta 1 (TGF-beta 1) was used to stimulate LX-2 cells. Results: Oridonin significantly inhibited LX-2 and HSC-T6 proliferation. In contrast, oridonin had no antiproliferative effect on C3A cells at our tested range. Oridonin induced apoptosis and S-phase arrest in LX-2 cells. These findings were associated with an increase in p53, p21, p16, and cleaved Poly (ADP-ribose) Polymerase (PARP), and with a decrease in Cyclindependent kinase 4 (Cdk4). Oridonin markedly decreased expression of alpha smooth muscle actin and ECM protein type I collagen and fibronectin, blocked TGF-beta 1-induced Smad2/3 phosphorylation and type I collagen expression. Conclusions: Oridonin induces apoptosis and cell cycle arrest involving the p53-p21 pathway in HSC and appears to be nontoxic to hepatocytes. In addition, oridonin suppressed endogenous and TGF-beta 1-induced ECM proteins. Thus, oridonin may act as a novel agent to prevent hepatic fibrosis. (C) 2014 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据