期刊
JOURNAL OF SURGICAL RESEARCH
卷 179, 期 1, 页码 87-93出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jss.2012.08.052
关键词
Colorectal cancer; Orthotopic mouse models; Green fluorescent protein; Red fluorescent protein; Fluorescence-guidance; Resection; Survival
类别
资金
- NCI NIH HHS [CA142669, T32 CA121938, CA121938-5, R33 CA109949, R01 CA142669, CA132971, R01 CA132971, R21 CA109949] Funding Source: Medline
Background: We inquired if fluorescence-guided surgery (FGS) could improve surgical outcomes in fluorescent orthotopic nude mouse models of human colon cancer. Methods: We established fluorescent orthotopic mouse models of human colon cancer expressing a fluorescent protein. Tumors were resected under bright light surgery (BLS) or FGS. Pre- and post-operative images with the OV-100 Small Animal Imaging System (Olympus Corp, Tokyo Japan) were obtained to assess the extent of surgical resection. Results: All mice with primary tumor that had undergone FGS had complete resection compared with 58% of mice in the BLS group (P = 0.001). FGS resulted in decreased recurrence compared with BLS (33% versus 62%, P = 0.049) and lengthened disease-free median survival from 9 to >36 wk. The median overall survival increased from 16 wk in the BLS group to 31 weeks in the FGS group. FGS resulted in a cure in 67% of mice (alive without evidence of tumor at >6 mo after surgery) compared with only 37% of mice that underwent BLS (P = 0.049). Conclusions: Surgical outcomes in orthotopic nude mouse models of human colon cancer were significantly improved with FGS. The present study can be translated to the clinic by various effective methods of fluorescently labeling tumors. (C) 2013 Elsevier Inc. All rights reserved.
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