期刊
JOURNAL OF SURGICAL RESEARCH
卷 167, 期 2, 页码 E137-E143出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jss.2010.12.031
关键词
MMP-7; Matrilysin; antibiotics; radiation; microflora; TGF-beta; MIP-2
类别
资金
- Cancer Foundation of Sweden. A. Pahlsson's Foundation
- Ruth and Richard Juhlin's Foundation, Lundgren's Foundation
- Gunnar Nilsson's Foundation, Einar and Inga Nilsson's Foundation
- Malmo University Hospital, Sweden
Background. Matrilysin (MMP-7) elevation after radiotherapy is shown in humans. Matrilysin regulates certain cytokines and the production of bactericidal proteins when the mucosa is exposed to bacterial antigens. We investigate the effect of irradiation on matrilysin and microflora in murine bowel, after modulation with antibiotics. Methods. Animals were divided into two different groups a radiation group (72 animals) and sham radiation group (36 animals). Animals were divided into smaller groups of six according to radiation dose (19 or 38 Gy or sham). Seven days before radiotherapy ampicillin 500 mg/kg/d was administered intramuscularly, in the antibiotic groups. An exteriorized segment of ileum was subjected to single high dose radiation (19 or 38 Gy). Samples were collected 2, 24, and 48 h and analyzed for microflora, MIP-2, TGF-beta, and MMP-7. Results. The combination of antibiotics and irradiation leads to an early significant reduction of bacteria, down- regulates MIP-2, up-regulates TGF-beta and elevation of MMP-7 to levels achieved by antibiotics or irradiation alone. Lactobacilli were reduced to non-existent levels after antibiotics. Conclusions. Pretreatment with Ampicillin before irradiation and laparotomy in a murine model leads to Matrilysin over-expression as achieved by radiotherapy alone. Microfloral regulation does not affect MMP-7 stimulation after surgical or radiological trauma. Radiotherapy overrides the effect of antibi-otics leading to an up-regulation of MMP-7, TGF-beta and MIP-2 expression between 24 h and 48 h. (C) 2011 Elsevier Inc. All rights reserved.
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