期刊
JOURNAL OF SURGICAL ONCOLOGY
卷 107, 期 2, 页码 201-205出版社
WILEY-BLACKWELL
DOI: 10.1002/jso.23214
关键词
microRNA; neoplasm; targeting
资金
- National High Technology Research and Development Program of China (863 Program) [2009AA022707]
- Promotion Program for Shenzhen Key Laboratory, Shenzhen, China [CXB200903090055A, CXB201005250016A]
- Bank of Clinical Data of Major Diseases and Biological Specimens of Shenzhen [CXC201005260001A]
Background and Objectives MicroRNAs (miRNAs) are aberrantly expressed in cancers. Dicer, Drosha, and Exportin 5 are essential for miRNA processing. In this study, the expression patterns of Dicer, Drosha, and Exportin 5 and the cell proliferation inhibition and apoptosis induced by silencing these genes in urothelial carcinoma of the bladder were determined. Methods The expression levels of Dicer, Drosha, and Exportin 5 were determined using Real-Time qPCR in 40 patients with urothelial carcinoma of the bladder. Bladder urothelial carcinoma T24 and 5637 cells were transfected with Dicer, Drosha, or Exportin 5 siRNA or negative control siRNA. Cell proliferation was determined using MTT assay. Apoptosis was evaluated using ELISA assay. Results All the three genes were up-regulated in bladder urothelial carcinoma compared to matched normal urothelium. Dicer, Drosha, and Exportin 5 expression levels were higher in high grade carcinomas than that in low grade carcinomas. Invasive carcinomas had higher expression levels than non-invasive carcinomas. Silencing Dicer, Drosha, or Exportin 5 induced cell proliferation inhibition and apoptosis in bladder urothelial carcinoma T24 and 5637 cells. Conclusions Dicer, Drosha, and Exportin 5 are promising biomarkers and therapeutic targets for urothelial carcinoma of the bladder. J. Surg. Oncol. 2013;107:201205. (c) 2012 Wiley Periodicals, Inc.
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