期刊
JOURNAL OF SURGICAL ONCOLOGY
卷 107, 期 5, 页码 555-559出版社
WILEY-BLACKWELL
DOI: 10.1002/jso.23264
关键词
lincRNA; taurine up-regulated gene 1; bladder cancer; proliferation; apoptosis
资金
- National High Technology Research and Development Program of China [2009AA022707]
- Promotion Program for Shenzhen Key Laboratory, Shenzhen, China [CXB201005250016A]
- Bank of Clinical Data of Major Diseases and Biological Specimens of Shenzhen [CXC201005260001A]
- National High Technology Research and Development Program of China (863 Program) [2009AA022707]
Background and Objectives Long intergenic non-coding RNAs (lincRNAs) are a class of non-coding RNAs that regulate gene expression via chromatin reprogramming. Taurine Up-regulated Gene 1 (TUG1) is a lincRNA that is associated with chromatin-modifying complexes and plays roles in gene regulation. In this study, we determined the expression patterns of TUG1 and the cell proliferation inhibition and apoptosis induced by silencing TUG1 in urothelial carcinoma of the bladder. Methods The expression levels of TUG1 were determined using Real-Time qPCR in a total of 44 patients with bladder urothelial carcinomas. Bladder urothelial carcinoma T24 and 5637 cells were transfected with TUG1 siRNA or negative control siRNA. Cell proliferation was evaluated using MTT assay. Apoptosis was determined using ELISA assay. Results TUG1 was up-regulated in bladder urothelial carcinoma compared to paired normal urothelium. High TUG1 expression levels were associated with high grade and stage carcinomas. Cell proliferation inhibition and apoptosis induction were observed in TUG1 siRNA-transfected bladder urothelial carcinoma T24 and 5637 cells. Conclusions Our data suggest that lincRNA TUG1 is emerging as a novel player in the disease state of bladder urothelial carcinoma. TUG1 may have potential roles as a biomarker and/or a therapeutic target in bladder urothelial carcinoma. J. Surg. Oncol. 2013;107:555559. (c) 2012 Wiley Periodicals, Inc.
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