期刊
JOURNAL OF SUPERCRITICAL FLUIDS
卷 55, 期 1, 页码 169-175出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.supflu.2010.07.002
关键词
Supercritical fluid extraction; Nannochloropsis oculata; Anti-solvent purification; Carotenoids; Zeaxanthin
资金
- National Science Council of the Republic of China, Taiwan [NSC97-2622-E005-011-CC2, NSC98-2221-E005-053-MY3]
- Taichung Veterans General Hospital
- National Chung Hsing University, Taiwan [TCVGH-NCHU 967603]
- Ministry of Education, Taiwan, ROC
This study investigated co-solvent modified supercritical carbon dioxide extraction of lipids and carotenoids from the microalgal species of Nannochloropsis oculata. Supercritical carbon dioxide (SCCO2) anti-solvent precipitation of carotenoids from the extracts following purification of Zeaxanthin was also examined. Continuous modification by ethanol of supercritical carbon dioxide extractions showed that the addition ratio was important for extraction efficiency of lipids and carotenoids. SCCO2 extraction at 350 bar, 323 K and 16.7 wt% of ethanol addition yielded 239.7 mg of triglycerides and 7.61 mg of carotenoids per gram extract with a total yield of 15.5%. SCCO2 anti-solvent experiments showed that the content of Zeaxanthin in the precipitate was greater than that in the fraction of normal phase column chromatography. The purest Zeaxanthin (93.8%) was then successfully isolated from the purified fraction by using a reverse-phase HPLC column chromatography. Rat macrophages treated by ultra-sonicated water extracts of the SCCO2 defatted algae showed a positive phagocytotic activity. (C) 2010 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据