期刊
PLANT SCIENCE
卷 230, 期 -, 页码 23-32出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2014.10.001
关键词
Rice seeds; Phosphate transporter; Phosphorus homeostasis; Source and sink organs
资金
- China 973 Program [2011CB100302]
- China National Natural Science Foundation
- National Program on R&D of Transgenic Plants, PAPD project in Jiangsu Province, Innovative Research Team Development Plan of the Ministry of Education of China
- 111 Project [B12009]
Phosphorus (P) redistribution from source to sink organs within plant is required for optimizing growth and development under P deficient condition. In this study, we knocked down expression of a phosphate transporter gene OsPht1;8 (OsPT8) selectively in shoot and/or in seed endosperm by RNA-interference using RISBZ1 and GluB-1 promoter (designate these transgenic lines as SSRi and EnSRi), respectively, to characterize the role of OsPT8 in P redistribution of rice. In comparison to wild type (WT) and EnSRi lines, SSRi lines under P deficient condition accumulated more Pin old blades and less P in young blades, corresponding to attenuated and enriched transcripts of P-responsive genes in old and young blades, respectively. The ratio of total P in young blades to that in old blades decreased from 2.6 for WT to 0.9-1.2 for SSRi lines. During the grain-filling stage, relative to WT, SSRi lines showed the substantial decrease of total P content in both endosperm and embryo, while EnSRi lines showed 40-50% decrease of total P content in embryo but similar P content in endosperm. Taken together, our results demonstrate that OsPT8 plays a critical role in redistribution of P from source to sink organs and P homeostasis in seeds of rice. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
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