期刊
JOURNAL OF STRUCTURAL BIOLOGY
卷 184, 期 3, 页码 385-393出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2013.10.016
关键词
DQE; MTF; Direct electron detector; Transmission electron microscopy
资金
- National Center for Research Resources [2 P41 RR017573]
- National Institute of General Medical Sciences from the National Institutes of Health [9 P41 GM103310]
- National Institutes of Health [P01 GM62580, T32-GM007596]
A new generation of direct electron detectors for transmission electron microscopy (TEM) promises significant improvement over previous detectors in terms of their modulation transfer function (MTF) and detective quantum efficiency (DQE). However, the performance of these new detectors needs to be carefully monitored in order to optimize imaging conditions and check for degradation over time. We have developed an easy-to-use software tool, FindDQE, to measure MTF and DQE of electron detectors using images of a microscope's built-in beam stop. Using this software, we have determined the DQE curves of four direct electron detectors currently available: the Gatan K2 Summit, the FEI Falcon I and II, and the Direct Electron DE-12, under a variety of total dose and dose rate conditions. We have additionally measured the curves for the Gatan US4000 and TVIPS TemCam-F416 scintillator-based cameras. We compare the results from our new method with published curves. (C) 2013 Elsevier Inc. All rights reserved.
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