期刊
JOURNAL OF STRUCTURAL BIOLOGY
卷 177, 期 2, 页码 179-192出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2011.11.025
关键词
Soft X-ray tomography; Mammalian cells; Ultrastructure
资金
- Human Frontier Science Program [RGP0053/2005-C]
- German Federal Ministry of Education and Research [05KS4BY1/7]
- National Institutes of Health, National Institute of Arthritis and Musculoskeletal and Skin Diseases
- National Cancer Institute, Center for Cancer Research [HHSN26120080001E]
We provide a catalog of 3D cryo soft X-ray tomography (cryo-SXT) images obtained from similar to 6 to 12 mu m thick mouse adenocarcinoma cells. Included are multiple representative images of nuclei, nucleoli, nuclear membrane, nuclear membrane channels, mitochondria, lysosomes, endoplasmic reticulum, filaments and plasma membrane, plus three structures not previously described by cryo-SXT, namely Golgi, microvilli and nuclear-membrane blebs. Sections from the 3D cryo-SXT tomograms for all the preceding structures closely resemble those seen by thin-section transmission electron microscopy (TEM). Some structures such as nuclear-membrane channels and nuclear-membrane blebs are more easily detected by cryo-SXT than TEM most likely due to their better contrast and cellular preservation in cryo-SXT combined with the ability to rapidly locate these structures within a full 3D image. We identify and discuss two current limitations in cryo-SXT: variability in image quality and difficulties in detecting weaker contrast structures such as chromatin and various nuclear bodies. Progress on these points is likely to come from the solution of several technical problems in image acquisition, plus the implementation of advanced cryo soft X-ray microscopy approaches such as phase contrast or optical sectioning. Published by Elsevier Inc.
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