Additional evidence against jasmonate-induced jasmonate induction hypothesis

Jasmonates are phytohormones involved in development and stress reactions. The most prominent jasmonate is jasmonic acid, however, the bioactive jasmonate is (+)-7-iso-jasmonoyl-L-isoleucine (JA-Ile). Biosynthesis of jasmonates is long time known; compartmentalization, enzymes and corresponding genes are well studied. Because all genes encoding these biosynthetic enzymes are jasmonate inducible, a hypothesis of jasmonate-induced-jasmonate-biosynthesis is widely accepted. Here, this hypothesis was revisited by employing the synthetic JA-Ile mimic coronalon to intact and wounded leaves, which excludes structural cross-contamination with endogenous jasmonates. At an effective concentration that induced various jasmonate-responsive genes in Arabidopsis, neither accumulation of endogenous jasmonic acid, JA-Ile, nor of their hydroxylated metabolites was detected. Results indicate that in spite of jasmonate-induced biosynthetic gene expression, no jasmonate biosynthesis/accumulation takes place supporting a post-translational regulation. (C) 2015 Elsevier Ireland Ltd. All rights reserved.