期刊
JOURNAL OF STRUCTURAL BIOLOGY
卷 180, 期 1, 页码 132-142出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2012.05.019
关键词
Electron tomography; Denoising; Plasmodium; Non-linear anisotropic diffusion filtering
资金
- Australian Research Council
- Australian National Health
- Medical Research Council
- Freddy Frischknecht and Marek Cyrklaff (University of Heidelberg, Germany)
- Wolfgang Baumeister (Max Planck for Biochemistry, Munich, Germany)
Electron tomography produces highly magnified 3D image volumes useful for investigating the structure and function of cellular components. Image quality is degraded by multiple scattering events and quantum noise, which depend on the angle at which individual tilt projections are collected. We have adapted a biomedical imaging approach to improve image quality by enhancing individual tilt projections prior to volumetric reconstruction. Specifically, we have developed a family of non-linear anisotropic diffusion (NAD) filters parameterized by the tilt angle. We give a quantitative and qualitative evaluation of our pre-processing approach and the NAD filter. We show an improvement in the reconstructed volumes for tomograms generated from both plastic-embedded and cryo-stabilized samples of malaria parasite-infected erythrocytes. (C) 2012 Elsevier Inc. All rights reserved.
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