期刊
JOURNAL OF STRUCTURAL BIOLOGY
卷 174, 期 1, 页码 234-238出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2010.10.002
关键词
Graphene; Graphene oxide; Cryo-EM; Electron microscopy; DNA; Transmission electron microscopy
资金
- Swiss National Science foundation (SystemsX.ch)
- DARPA iMINT centre
- DARPA CERA centre
- Office of Naval Research
- Advanced Energy Consortium
- German Research Foundation (DFG)
- Ministry of Science, Research and Arts (MWK) of Baden-Wurttemberg
This technical note describes the transfer of continuous, single-layer, pristine graphene to standard Quantifoil TEM grids. We compare the transmission properties of pristine graphene substrates to those of graphene oxide and thin amorphous carbon substrates. Positively stained DNA imaged across amorphous carbon is typically indiscernible and requires metal shadowing for sufficient contrast. However, in a practical illustration of the new substrates properties, positively stained DNA is imaged across pristine graphene in striking contrast without the need of metal shadowing. We go onto discuss technical considerations and the potential applications of pristine graphene substrates as well as their ongoing development. (C) 2010 Elsevier Inc. All rights reserved.
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