期刊
JOURNAL OF STRUCTURAL BIOLOGY
卷 167, 期 1, 页码 19-24出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2009.03.012
关键词
Basement membrane; Cornea; Modulus; Atomic force microscopy (AFM)
资金
- National Eye Institute [5R01EY016134-02, 1R01CA133567-01]
- National Science Foundation [DMR-632527]
Biophysical cues such as substrate modulus have been shown to influence a variety of cell behaviors. We have determined the elastic modulus of the anterior basement membrane and Descemet's membrane of the human cornea with atomic force microscopy (AFM). A spherical probe was used with a radius approximating that of a typical cell focal adhesion. Values obtained for the elastic modulus of the anterior basement membrane range from 2 to 15 kPa, with a mean of 7.5 +/- 4.2 kPa. The elastic modulus of Descemet's membrane was found to be slightly higher than those observed for the anterior basement membrane, with a mean of 50 +/- 17.8 kPa and a range of 20-80 kPa. The topography of Descemet's membrane has been shown to be similar to that of the anterior basement, but with smaller pore sizes resulting in a more tightly packed structure. This structural difference may account for the observed modulus differences. The determination of these values will allow for the design of a better model of the cellular environment as well as aid in the design and fabrication of artificial corneas. (C) 2009 Elsevier Inc. All rights reserved.
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