期刊
JOURNAL OF STRUCTURAL BIOLOGY
卷 166, 期 1, 页码 95-102出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2009.01.002
关键词
CryoEM; EM; TEM; Single particle analysis; 3D reconstruction; Image processing; Automation; Automated CryoEM; CryoEM pipeline
资金
- National Institutes of Health (NIH)
- National Center for Research Resources' P41 program [RR17573, RR023093]
- ARCS foundation
The use of cryoEM and three-dimensional image reconstruction is becoming increasingly common. Our vision for this technique is to provide a straightforward manner in which users can proceed from raw data to a reliable 3D reconstruction through a pipeline that both facilitates management of the processing steps and makes the results at each step more transparent. Tightly integrated with a relational SQL database, Appion is a modular and transparent pipeline that extends existing software applications and procedures. The user manages and controls the software modules via web-based forms, and all results are similarly available using web-based viewers directly linked to the underlying database, enabling even naive users to quickly deduce the quality of their results. The Appion API was designed with the principle that applications should be compatible with a broad range of specimens and that libraries and routines are modular and extensible. Presented here is a description of the design and architecture of the working Appion pipeline prototype and some results of its use. (C) 2009 Elsevier Inc. All rights reserved.
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