4.4 Article

Heparin binding induces conformational changes in Adeno-associated virus serotype 2

期刊

JOURNAL OF STRUCTURAL BIOLOGY
卷 165, 期 3, 页码 146-156

出版社

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2008.12.002

关键词

Adeno-associated virus; Receptor attachment; Parvovirus; Virus structure; Electron cryo-microscopy; 3D image reconstruction; Atomic modeling; Heparin sulfate

资金

  1. NIH NHLBI [P01 HL59412, P01 HL51811]
  2. NIH GM [R37-033050]
  3. NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [P01HL051811, P01HL059412] Funding Source: NIH RePORTER
  4. NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [P01AI045976] Funding Source: NIH RePORTER
  5. NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R37GM033050] Funding Source: NIH RePORTER

向作者/读者索取更多资源

Adeno-associated virus serotype 2 (AAV2) uses heparan sulfate proteoglycan as a cell surface-attachment receptor. In this study the structures of AAV2 alone and complexed with heparin were determined to similar to 18 angstrom resolution using cryo-electron microscopy and three-dimensional image reconstruction. A difference map showed positive density, modeled as heparin, close to the icosahedral twofold axes and between the protrusions that surround the threefold axes of the capsid. Regions of the model near the threefold place the receptor in close proximity to basic residues previously identified as part of the heparin binding site. The region of the model near the twofold axes identifies a second contact site, not previously characterized but which is also possibly configured by heparin binding. The difference map also revealed two significant conformational changes: (1) at the tops of the threefold protrusions, which have become flattened in the complex, and (11) at the fivefold axes where the top of the channel is widened possibly in response to movement of the HI loops in the capsid proteins. Ordered density in the interior of the capsid in the AAV2-heparin complex was interpreted as nucleic acid, consistent with the presence of non-viral DNA in the expressed capsids. (C) 2008 Elsevier Inc. All rights reserved.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.4
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据