期刊
PLANT SCIENCE
卷 238, 期 -, 页码 198-211出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2015.06.013
关键词
Aquaporins; Fruit; Major intrinsic proteins; Ripening; Strawberry; Water transport
资金
- Ministerio Espanol de Ciencia e Innovacion (MICINN) [BIO2010-19322]
Strawberry fruit (Fragaria x ananassa) is a soft fruit with high water content at ripe stage (more than 90% of its fresh weight). Aquaporins play an important role in plant water homeostasis, through the facilitation of water transport and solutes. We report the role played by FaNIP1;1 in the receptacle ripening process. The analysis by qRT-PCR of FaNIP1;1 showed that this gene is mainly expressed in fruit receptacle and has a ripening-related expression pattern that was accompanied by an increase in both the abscisic acid and water content of the receptacle throughout fruit ripening. Moreover, FaNIP1;1 was induced in situations of water deficit. Additionally, we show that FaNIP1;1 expression was positively regulated by abscisic acid and negatively regulated by auxins. The water transport capacity of FaNIP1;1 was determined by a stopped-flow spectroscopy in yeast over-expressing FaNIP1;1. Glycerol, H2O2 and boron transport were also demonstrated in yeast. On the other hand, GFP-FaNIP1;1 fusion protein was located in plasma membrane. In conclusion, FaNIP1;1 seems to play an important role increasing the plasma membrane permeability, that allows the water accumulation in the strawberry fruit receptacle throughout the ripening process. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
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