期刊
JOURNAL OF STRUCTURAL BIOLOGY
卷 163, 期 1, 页码 10-17出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2008.03.009
关键词
intermediate-like filament; flagella; motility; cell division; spirochetes; electron microscopy; septation
资金
- NCRR NIH HHS [P41 RR001219-257228, P41 RR001219-266267, P41 RR001219-210043, P41 RR001219-200043, P41 RR001219, P41 RR01219, P41 RR001219-230084, P41 RR001219-266252, P41 RR001219-230096, P41 RR001219-180043, P41 RR001219-19A1S10043, P41 RR001219-257214, P41 RR001219-19A10043, P41 RR001219-27] Funding Source: Medline
- NIAID NIH HHS [R01 AI034354-07, R01 AI034354-09, R01 AI034354-06A1, AI35354, R01 AI034354-08] Funding Source: Medline
- NIDCR NIH HHS [DE07106, R21 DE017106-02, R21 DE017106-01A1, R21 DE017106] Funding Source: Medline
Using cryo-electron tomography, we are developing a refined description of native cellular structures in the pathogenic spirochete Treponema denticola. Tightly organized bundles of periplasmic flagella were readily observed in intact plunge-frozen cells. The periplasmic space was measured in both wild-type and aflagellate strains, and found to widen by less than the diameter of flagella when the latter are present. This suggests that a structural change occurs in the peptidoglycan layer to accommodate the presence of the flagella. In dividing cells, the flagellar filaments were found to bridge the cytoplasmic cylinder constriction site. Cytoplasmic filaments, adjacent to the inner membrane, run parallel to the tightly organized flagellar filaments. The cytoplasmic filaments may be anchored by a narrow plate-like structure. The tapering of the cell ends was conserved between cells, with a patella-shaped structure observed in the periplasm at the tip of each cytoplasmic cylinder. Several incompletely characterized structures have been observed in the periplasm between dividing cells, including a cable-like structure linking two cytoplasmic cylinders and complex foil-shaped structures. (C) 2008 Elsevier Inc. All rights reserved.
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