期刊
JOURNAL OF STRUCTURAL BIOLOGY
卷 161, 期 2, 页码 211-218出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2007.10.015
关键词
Zernike phase plate; single particle reconstruction; phase contrast; GroEL; cryomicroscopy
We present the first application of Zernike phase-contrast transmission electron microscopy to single-particle 3D reconstruction of a protein, using GroEL chaperonin as the test specimen. We evaluated the performance of the technique by comparing 3D models derived from Zernike phase contrast imaging, with models from conventional underfocus phase contrast imaging. The same resolution, about 12 angstrom, was achieved by both imaging methods. The reconstruction based on Zernike phase contrast data required about 30% fewer particles. The advantages and prospects of each technique are discussed. (C) 2007 Elsevier Inc. All rights reserved.
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