期刊
JOURNAL OF STORED PRODUCTS RESEARCH
卷 56, 期 -, 页码 43-48出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jspr.2013.10.005
关键词
Wheat; Detection of infestation; Enzymes; Electrophoresis; Zymography
类别
资金
- Ministry of Science and Higher Education of the Republic of Poland [N N312 211 036]
Granary weevil infestation in wheat kernels is difficult to detect since the insects cause a hidden infestation by developing inside the grain. In order to identify an early infestation of stored wheat, a technique involving electrophoresis combined with zymographic assay of alpha-amylases has been developed. The concept of the method is based on the differences between the electrophoretic mobility of amylases present in wheat kernels and those secreted by the insect's digestive system. The diagnostic accuracy of the method developed was compared to expert scoring, based on microscopical observation. It is proposed that zymographic assay of alpha-amylases, which does not require either expensive equipment or chemicals, is a suitable method for the accurate detection of granary weevil alpha-amylase even if beetles are only present on grain for a very short period of time such as one day. (C) 2013 Elsevier Ltd. All rights reserved.
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