Mechanism of inhibitory action of Eldecalcitol, an active vitamin D analog, on bone resorption in vivo

Bone-resorbing osteoclasts differentiate from hematopoietic precursors under the strict regulation of bone-forming osteoblasts. Osteoblasts express two cytokines essentially required for osteoclastogenesis; macrophage-colony stimulating factor (M-CSF) and receptor activator of nuclear factor kappa B ligand (RANKL). Osteoblasts express constitutively M-CSF, and inducibly RANKL in response to bone resorption-stimulating factors. The active form of vitamin D3, 1 alpha,25-dihydroxyvitamin D3 [1 alpha,25(OH)(2)D-3], is known to be a hormone which enhances RANKL expression in vitro. Nevertheless, Calcitoriol [1 alpha,25(OH)(2)D-3] and its prodrug, Alfacalcidol (1 alpha-hydroxyvitamin D-3) have been taken as therapeutic drugs in osteoporotic patients in Japan. In addition, Eldecalcitol [2 beta-(3-hydroxypropoxy)-1 alpha,25(OH)(2)D-3], a new analog of 1 alpha,25(OH)(2)D-3, was approved as a therapeutic agent for osteoporosis in Japan in 2011. Interestingly, those vitamin D compounds increased bone mineral density due to the suppression of bone resorption in vivo. We previously showed that cycle-arrested quiescent osteoclast precursors (QOPs) were the direct osteoclasts precursors in vivo. We then investigated effects of daily administration of Eldecalcitol on bone resorption in mice. Bone mineral density was increased through the suppression of RANKL expression in osteoblasts in mice treated with Eldecalcito. The number of QOPs remained unchanged in bone. These results suggest that a long-term exposure of osteoblasts to vitamin D compounds down-regulate RANKL expression. This article is part of a Special Issue entitled '15th Vitamin D Workshop'. (C) 2012 Elsevier Ltd. All rights reserved.