期刊
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
卷 112, 期 1-3, 页码 20-24出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jsbmb.2008.07.004
关键词
Colorectal cancer; Estrogen receptor alpha variant; Quantitative real-time PCR
资金
- National Natural Science Foundation of China [30772510]
- Ministry Health of The People's Republic of China [WKJ2006-2-008]
- Department of Science and Technology Zhe Jiang Province [2007C24011]
Estrogen is involved in suppression of colorectal cancer development and exerts its function via estrogen receptors a, (3 and their splicing variants. Whether the recently indentified ER-alpha splicing variants, ER-alpha 36 and ER-alpha 46, play a role in colorectal cancer development is unknown. In this study, we quantified the mRNA copy numbers of wild type ER-alpha (ER-alpha 66), ER-alpha 46 and ER-alpha 36 in 35 colorectal cancers and their matched normal colorectal tissues by quantitative real-time PCR assay, and correlated their mRNA levels with the clinicopathological properties of the tumors. We found that ER-alpha 66, ER-alpha 46 and ER-alpha 36 mRNAs were coexpressed in all colorectal cancers and their matched normal tissues. The decreased mRNA levels of ER-alpha 36 and ER-alpha 46 whereas no difference of ER-alpha 66 mRNA was observed in colorectal cancers compared to their matched normal tissues. Moreover, change in the expression of ER-alpha 36 mRNA level was correlated with Dukes' stage of the tumor and the lymph node metastasis. ER-alpha 36 mRNA was decreased significantly in Dukes' C+D compared to Dukes' A+B stage tumors (P=0.017), and the expression of ER-alpha 36 mRNA in N-1/N-2 was lower than that in No lymph node metastasis (P=0.049). So ER-alpha 36 and ER-alpha 46 might be implicated in the development and progression of colorectal cancers. (C) 2008 Elsevier Ltd. All rights reserved.
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