期刊
PLANT PATHOLOGY
卷 64, 期 6, 页码 1284-1291出版社
WILEY
DOI: 10.1111/ppa.12366
关键词
begomovirus; Bemisia tabaci; seed transmission; sweet potato; Sweet potato leaf curl virus
资金
- Agricultural Biotechnology Development Program, Ministry of Agriculture, Food and Rural Affairs of Republic of Korea [311058-05-3-HD140, 110034-05-4-HD110]
Sweet potato leaf curl virus (SPLCV) infects sweet potato and is a member of the family Geminiviridae (genus Begomovirus). SPLCV transmission occurs from plant to plant mostly via vegetative propagation as well as by the insect vector Bemisia tabaci. When sweet potato seeds were planted and cultivated in a whitefly-free greenhouse, some sweet potato plants started to show SPLCV-specific symptoms. SPLCV was detected by PCR from all leaves and floral tissues that showed leaf curl disease symptoms. More than 70% of the seeds harvested from SPLCV-infected sweet potato plants tested positive for SPLCV. SPLCV was also identified from dissected endosperm and embryos. The transmission level of SPLCV from seeds to seedlings was up to 15%. Southern blot hybridization showed SPLCV-specific single-and double-stranded DNAs in seedlings germinated from SPLCV-infected seeds. Taken altogether, the results show that SPLCV in plants of the tested sweet potato cultivars can be transmitted via seeds and SPLCV DNA can replicate in developing seedlings. This is the first seed transmission report of SPLCV in sweet potato plants and also, to the authors' knowledge, the first report of seed transmission for any geminivirus.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据