期刊
PLANT MOLECULAR BIOLOGY
卷 88, 期 4-5, 页码 401-413出版社
SPRINGER
DOI: 10.1007/s11103-015-0329-7
关键词
P-SARK::IPT; OsWRKY47; WRKY transcription factor; Delayed senescence; Drought tolerance
资金
- UC Office of the President, UC Discovery Grant [212976]
- Agencia Nacional de Promocion Cientifica y Tecnologica [PICT 2011 0850, PICT 2012 0955]
- Universidad Nacional del Litoral (UNL)
OsWRKY47 is a divergent rice transcription factor belonging to the group II of the WRKY family. A transcriptomic analysis of the drought response of transgenic rice plants expressing P (SARK) ::IPT, validated by qPCR, indicated that OsWRKY47 expression was induced under drought stress in P (SARK) ::IPT plants. A PCR-assisted site selection assay (SELEX) of recombinant OsWRKY47 protein showed that the preferred sequence bound in vitro is (G/T)TTGACT. Bioinformatics analyses identified a number of gene targets of OsWRKY47; among these two genes encode a Calmodulin binding protein and a Cys-rich secretory protein. Using Oswrk47 knockout mutants and transgenic rice overexpressing OsWRKY47 we show that the transcription of these putative targets were regulated by OsWRKY47. Phenotypic analysis carried out with transgenic rice plants showed that Oswrky47 mutants displayed higher sensitivity to drought and reduced yield, while plants overexpressing OsWRKY47 were more tolerant.
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