期刊
PLANT JOURNAL
卷 85, 期 1, 页码 96-106出版社
WILEY-BLACKWELL
DOI: 10.1111/tpj.13092
关键词
WRKY71; transcription factor; flowering; FLOWERING LOCUS T; LEAFY; Arabidopsis thaliana
资金
- National Special Science Research Program of China [2013CB967300]
- National High Technology Research and Development Program '863' [2013AA102602-4]
- National Transgenic Project of China [2013ZX08010002-002]
- National Natural Science Foundation [30970243, 31270328, 31471515, 31201269, 31210103901, 31500232]
- China Postdoctoral Science Foundation funded project [2014M551893, 2015M572014]
- Science and Technology Plan of Shandong Province [2013GNC11010]
- Promotive Research Fund for Excellent Young and Middle-aged Scientists of Shandong Province [BS2013SW027]
- Research Program for International SAMP
- T Cooperation Projects of Shandong Province [2011176]
- Shandong Province Natural Science Foundation [ZR2014CP002]
- State Key Laboratory of Plant Genomics grant [2011B0525]
Flowering is crucial for achieving reproductive success. A large number of well-delineated factors affecting flowering are involved in complex genetic networks in Arabidopsis thaliana. However, the underlying part played by the WRKY transcription factors in this process is not yet clear. Here, we report that WRKY71 is able to accelerate flowering in Arabidopsis. An activation-tagged mutant WRKY71-1D and a constitutive over-expresser of WRKY71 both flowered earlier than the wild type (WT). In contrast, both the RNA interference-based multiple WRKY knock-out mutant (w71w8 + 28RNAi) and the dominant repression line (W71-SRDX) flowered later. Gene expression analysis showed that the transcript abundance of the flowering time integrator gene FLOWERING LOCUS T (FT) and the floral meristem identity genes LEAFY (LFY), APETALA1 (AP1) and FRUITFULL (FUL) were greater in WRKY71-1D than in the WT, but lower in w71w8 + 28RNAi and W71-SRDX. Further, WRKY71 was shown to bind to the W-boxes in the FT and LFY promoters in vitro and in vivo. The suggestion is that WRKY71 activity hastens flowering via the direct activation of FT and LFY.
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