期刊
PLANT JOURNAL
卷 84, 期 4, 页码 704-717出版社
WILEY
DOI: 10.1111/tpj.13033
关键词
Chlamydomonas reinhardtii; codon usage; GC content; translation; RNA stability; epigenetics; histone modification
资金
- Max Planck Society
- Ministry of Science, Research and Technology of Iran
- EMBO fellowship
- Deutsche Forschungsgemeinschaft
- Bundesministerium fur Bildung und Forschung
- National Institutes of Health [GM42143]
The efficiency of gene expression in all organisms depends on the nucleotide composition of the coding region. GC content and codon usage are the two key sequence features known to influence gene expression, but the underlying molecular mechanisms are not entirely clear. Here we have determined the relative contributions of GC content and codon usage to the efficiency of nuclear gene expression in the unicellular green alga Chlamydomonas reinhardtii. By comparing gene variants that encode an identical amino acid sequence but differ in their GC content and/or codon usage, we show that codon usage is the key factor determining translational efficiency and, surprisingly, also mRNA stability. By contrast, unfavorable GC content affects gene expression at the level of the chromatin structure by triggering heterochromatinization. We further show that mutant algal strains that permit high-level transgene expression are less susceptible to epigenetic transgene suppression and do not establish a repressive chromatin structure at the transgenic locus. Our data disentangle the relationship between GC content and codon usage, and suggest simple strategies to overcome the transgene expression problem in Chlamydomonas.
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