A New Approach for Thermodynamic Studies on Binding of Some Metal Ions with Human Growth Hormone

A thermodynamic study on the interaction between Mg2+, Ni2+ and Co2+ ions (M2+) and human growth hormone ( hGH) was made at 27 degrees C in aqueous NaCl solutions using isothermal titration calorimetry. Gholamreza Rezaei Behbehani's solvation model (GRB) was used to model the enthalpies of M2+ + hGH interactions over the studied range of metal ion concentrations. The solvation parameters derived from the solvation model were attributed to a structural change of hGH due to its interactions with the metal ion.