Direct electrochemical analysis of glucose oxidase on a graphene aerogel/gold nanoparticle hybrid for glucose biosensing

Homogeneously distributed self-assembling graphene aerogel/gold nanoparticle (GA/GNs) hybrid materials with three-dimensional (3D) interconnected pores were fabricated through a simple hydrothermal route. A highly sensitive glucose oxidase (GOD) sensor is then developed based on the GA/GN hybrid materials. The obtained porous structure of the GA/GNs hybrid favored high-density immobilization of the enzyme and penetration of water-soluble molecules, which provided a biocompatible sensing platform for GOD immobilization. This GOD biosensor exhibits remarkable sensitivity (257.60 mu A center dot mM(-1) center dot cm(-2)), an approximate linear detection range of glucose concentration (50 mu mol center dot L-1 to 450 mu mol center dot L-1), and a detection limit of 0.597 mu mol center dot L-1. These results indicate that the in situ encapsulation of different nanomaterials into a 3D graphene aerogel framework can enable the fabrication of a series of graphene-based 3D porous materials with promising applications.