期刊
PLANT CELL TISSUE AND ORGAN CULTURE
卷 121, 期 2, 页码 397-412出版社
SPRINGER
DOI: 10.1007/s11240-015-0711-9
关键词
Grapevine; Somatic embryogenesis; Transformation; Stilbene synthase; Powdery mildew
资金
- National Natural Science Foundation of China [31372039]
- Program for Innovative Research Team of Grape Germplasm Resource and Breeding [2013KCT-25]
To facilitate genetic improvement, an efficient system of embryogenic culture induction, maintenance and transformation in Vitis vinifera cv. Chardonnay was developed using picloram. Whole flowers produced the most embryogenic calluses on induction media containing Murashige and Skoog's (MS) basal salts and 3.0 mg L-1 picloram with different concentrations of 2,4-dichlorophenoxyacetic acid and 6-benzyladenine. Proembryonic masses (PEM) cultured on maintenance and proliferation media (MPM) containing MS basal salts and picloram had the highest proliferation efficiency within 2 months. Additionally, the best PEM formation was found with MPM containing MS basal salts and 2.0 mg L-1 picloram. Then this PEM cultured on MPM were transferred to embryo germination medium containing MS basal salts, 0.2 mg L-1 kinetin and 0.1 mg L-1 2-naphthoxyacetic acid, the optimal medium for normal plantlets regeneration. A single copy of the stilbene synthase gene VpSTSgDNA2 from Chinese wild Vitis pseudoreticulata was transferred into regenerated Chardonnay via Agrobacterium tumefaciens-mediated transformation and confirmed by Southern blot analysis. The positive transgenic grapevine lines exhibited higher levels of stilbene and H2O2 than wild-type vines and could slightly reduced the growth of powdery mildew.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据