期刊
PLANT CELL REPORTS
卷 34, 期 8, 页码 1401-1415出版社
SPRINGER
DOI: 10.1007/s00299-015-1796-7
关键词
Aquaporin; Atriplex canescens; Overexpression; Plant growth; Abiotic stress
资金
- 12th Five-Year Plan Project of Science and Technology Support, China [2012BAD19B04, 2014BAD14B02]
- Research and Development of Industrial Technology Special at Jilin Provincial Development and Reform Commission [2013C001]
- Ministry of Agriculture Key Project of GM Cultivation of New Varieties, P. R. China [2013ZX08004004]
- Graduate Innovation Fund of Jilin University [2014119]
An aquaporin protein AcPIP2 from Atriplex canescens was involved in plant growth rate, abiotic stress tolerance in Arabidopsis. Under limited water condition, AcPIP2 leaded to the sensitivity to drought stress. An aquaporin protein (AcPIP2) was obtained from the saltbush Atriplex canescens, which was in PIP2 subgroup belonging to the PIP subfamily, MIP superfamily. The subcellular localization of AcPIP2 showed the fusion protein AcPIP2-eGFP located at the plasma membrane in Nicotiana benthamiana. Overexpression of AcPIP2 in Arabidopsis fully proved that AcPIP2 was involved in plant growth rate, transpiration rate and abiotic stress tolerance (NaCl, drought and NaHCO3) in Arabidopsis, which is mostly in correspondence to gene expression pattern characterized by qRT-PCR performed in A. canescens. And under limited water condition, AcPIP2 overexpression leaded to the sensitivity to drought stress. In the view of the resistant effect in transgenic Arabidopsis overexpressing AcPIP2, the AcPIP2 may throw some light into understanding how the A. canescens plants cope with abiotic stress, and could be used in the genetic engineering to improve plant growth or selective tolerance to the abiotic stress.
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