ASG2 is a farnesylated DWD protein that acts as ABA negative regulator in Arabidopsis

The tagging-via-substrate approach designed for the capture of manunal prenylated proteins was adapted to Arabidopsis cell culture. In this way, proteins are in vivo tagged with an azide-modified farnesyl moiety and captured thanks to biotin alkyne Click-in chemistry with further streptavidin-allifitv chromatography. Mass spectrometry analyses identified four small GTPases and ASG2 (ALTERED SEED GERMINATION 2), a protein previously associated to the seed germination gene network. ASG2 is a conserved protein in plants and displays a unique feature that associates WD40 domains and tetratricopeptide repeats. Additionally, we show that ASG2 has a C-terminal CaaX-box that is famesylated in vitro. Protoplast transfecfions using CaaX prenyltransferase mutants show that farnesylation provokes ASG2 nucleus exdusion. Moreover, ASG2 interacts with DDB1 (DAMAGE DNA BINDING protein 1), and the subcellular localization of this complex depends on ASG2 farnesylation status. Finally, germination and root elongation experiments reveal that asg2 and the farnesyltransferase mutant eral (ENHANCED RESPONSE TO ABSCISIC ACID (ABA) 1) behave in similar manners when exposed to ABA or salt stress. To our knowledge, ASG2 is the frst farnesylated DWD (DDB1 binding WD40) protein related to ABA response in Arabidopsis that may be linked to eral phenotypes.